Stephen King said of the film, "THE AUTOPSY OF JANE DOE: Visceral horror to rival ALIEN and early Cronenberg. Watch it, but not alone."
Martin Sheen was originally cast as Tommy, but had to drop out due to scheduling conflicts. He was replaced by Brian Cox.
The song playing throughout this movie is "Open up your heart (and let the sun shine in)" performed by the Cowboy Church Sunday School. The version used is the 45 rpm recording played at 33 1/3.
The names "John Doe" or "John Roe" for men, "Jane Doe" or "Jane Roe" for women, "Johnny Doe" and "Janie Doe" for children, or just "Doe" non-gender-specifically are used as placeholder names for a party whose true identity is unknown or must be withheld in a legal action, case, or discussion
The director's favorite scene to film was the scene in the elevator when they discuss the death of the mother. He said "I only placed the camera, and we watched a fantastic performance by Brian Cox and Emile Hirsch". The scene he was most proud of as a director was the build up to the reveal of the symbols beneath Jane Doe's skin, as stated at the festival Monsters of Film in Stockholm 2016.